ega_script

The tool is used to:

• Generate the jobs for ega-file-transfer-to-collab
• Audit and report the files to be staged to or removed from ega Aspera server

Getting Started

The tool needs to talk to two different kinds of git repository to gather the information to realize the above tasks.

• The ega auditing git repository(https://github.com/icgc-dcc/ega-file-transfer.git)
• Job repositories which are tracking ega-file-transfer-to-collab job status(e.g., http://142.1.177.124/jt-hub/ega-file-transfer-to-collab-jtracker)

Prerequisites

Before you can run the tool, you need to configure the tool. The configuration file locates ega_script/conf/conf.yaml. You may need to change the following two base_path for ega_audit and ega_job respectively.

ega_audit_base_path: "../ega-file-transfer"
ega_job_base_path: ".."

The above default configuration will assume:

• All the job repos which are set up for ega-file-transfer-to-collab, e.g., ega-file-transfer-to-collab-jtracker(http://142.1.177.124/jt-hub/ega-file-transfer-to-collab-jtracker), are git clone to the same folder as the tool script
• The ega auditing git repository: ega-file-transfer(https://github.com/icgc-dcc/ega-file-transfer) is also git clone to the same folder as the tool script

├── ega-file-transfer
├── ega-file-transfer-to-collab-2-jtracker
├── ega-file-transfer-to-collab-3-jtracker
├── ega-file-transfer-to-collab-4-jtracker
├── ega-file-transfer-to-collab-5-jtracker
├── ega-file-transfer-to-collab-jtracker
└── ega_script

Since ega auditing git repository is version controlled, before we can run the tool to generate the jobs and report to_stage or to_remove files, we also need to set the version of the ega auditing reports in the conf/conf.yaml, e.g.,

file_version: "v20170630"

Installing

Get the source script of the tool

git clone [email protected]:lindaxiang/ega_script.git

Then you can run ./main.py -h to get the usage of the tool

usage: main.py [-h] [-c CONF] -t TASK [-p [PROJECT [PROJECT ...]]]
               [-s [SEQ_STRATEGY [SEQ_STRATEGY ...]]]

EGA-file-to-colllab job generator and auditor

optional arguments:
  -h, --help            show this help message and exit
  -c CONF, --setting CONF
                        Specify ega setting file
  -t TASK, --task TASK  Specify the task
  -p [PROJECT [PROJECT ...]], --project [PROJECT [PROJECT ...]]
                        Specify the project
  -s [SEQ_STRATEGY [SEQ_STRATEGY ...]], --seq_strategy [SEQ_STRATEGY [SEQ_STRATEGY ...]]
                        Specify the sequencing strategy

Running the tool to generate the jobs

For example generating jobs for RNA-Seq data of project CLLE-ES, do this:

cd ega_script
./main.py -t job -p CLLE-ES -s RNA-Seq

• If no project is specified, the tool will generate the eligible jobs for all the projects which have auditing reports available.
• If no seq_strategy is specified, the tool will generate the eligible jobs for all kinds of seq_strategy which are included in the related auditing reports.
• The generated jobs locates in job_state.backlog of one of the job repositories which is defined in the conf/conf.yaml, you can change the job folder if needed:

job_folder: "ega-file-transfer-to-collab-2-jtracker/ega-file-transfer-to-collab.0.6.jtracker/job_state.backlog"

Running the tool to generate the to_stage files

In order to get the list of files which are to be staged to Aspera server by EGA, do this:

cd ega_script
./main.py -t stage

You can specify the project and seq_strategy in order to get the list of files which are only for given sequence strategies and belong to given projects. The tool will generate to_stage_*.tsv files under each project. For example:

ega_operation/
├── BRCA-KR
│   └── to_stage_run.tsv
├── CLLE-ES
│   └── to_stage_run.tsv
├── LICA-FR
│   └── to_stage_run.tsv
├── MALY-DE
│   └── to_stage_analysis.tsv
├── OV-AU
│   └── to_stage_analysis.tsv
├── PACA-AU
│   ├── to_stage_analysis.tsv
│   └── to_stage_run.tsv
├── PAEN-AU
│   └── to_stage_analysis.tsv
└── to_remove.tsv

Running tool to generate the to_remove files

In order to list all files which can be removed from Aspera server by EGA, do this:

cd ega_script
./main.py -t remove

The tool will generate to_remove.txt file locating at: ega-file-transfer/ega_operation/to_remove.tsv

Log information

When using the tool to generate the jobs or report the to_stage or to_remove files, the tool did many QC checks based on the auditing reports, the QC results are logged into the *.log files locates:

ega_script/log/
├── error.log
├── info.log
└── warn.log

Here are some sample log messages:

2017-07-25 15:12:45,689 - audit.stage - WARNING - LICA-FR::EGAF00000483937 has the same file_md5sum and encrypted_file_md5sum: set(['772febc5f8fea25a9b09e43dd51e43bd'])
2017-07-25 15:12:45,690 - audit.stage - WARNING - LICA-FR::EGAF00000483938 has the same file_md5sum and encrypted_file_md5sum: set(['170588f8a583c2d4fee882fdfcb6133b'])
2017-07-25 15:12:45,690 - audit.stage - WARNING - LICA-FR::EGAF00000483899 has the same file_md5sum and encrypted_file_md5sum: set(['83aed772452945dc994bcfad7edebc3a'])
2017-07-25 15:12:49,248 - audit.stage - WARNING - MALY-DE::EGAF00001592148 has the id inconsistent: ega_analysis_id in audit report version v20170630
2017-07-25 15:12:49,248 - audit.stage - WARNING - MALY-DE::EGAF00001592148 has the id inconsistent: file_name in audit report version v20170630
2017-07-25 15:12:49,248 - audit.stage - WARNING - MALY-DE::EGAF00001592148 has the id inconsistent: encrypted_file_md5sum in audit report version v20170630

Authors

• Linda Xiang - Initial work