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@@ -22,13 +22,12 @@ Cellpose was written by Carsen Stringer and Marius Pachitariu. To learn about Ce
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Please see install instructions [below](README.md/#Installation), and also check out the detailed documentation at [**cellpose.readthedocs.io**](https://cellpose.readthedocs.io/en/latest/) for more information. Example notebooks:
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*[run_cellpose3.ipynb](https://github.com/MouseLand/cellpose/blob/main/notebooks/run_cellpose3.ipynb)[](https://colab.research.google.com/github/MouseLand/cellpose/blob/main/notebooks/run_cellpose3.ipynb) shows how to run image restoration using new `CellposeDenoiseModel` from Cellpose3
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*[run_cyto3.ipynb](https://github.com/MouseLand/cellpose/blob/main/notebooks/run_cellpose3.ipynb)[](https://colab.research.google.com/github/MouseLand/cellpose/blob/main/notebooks/run_cyto3.ipynb) shows how to use new super-generalist "cyto3" model with `model_type="cyto3"`.
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*[](https://colab.research.google.com/github/MouseLand/cellpose/blob/main/notebooks/run_cellpose_2.ipynb)
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Train your own models with Cellpose.
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*[run_cellpose_GPU.ipynb](https://github.com/MouseLand/cellpose/blob/main/notebooks/run_cellpose_GPU.ipynb)[](https://colab.research.google.com/github/MouseLand/cellpose/blob/main/notebooks/run_cellpose_GPU.ipynb) runs Cellpose segmentation in 2D and 3D
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*[Cellpose_cell_segmentation_2D_prediction_only.ipynb](https://github.com/MouseLand/cellpose/blob/main/notebooks/Cellpose_cell_segmentation_2D_prediction_only.ipynb)[](https://colab.research.google.com/github/MouseLand/cellpose/blob/main/notebooks/Cellpose_cell_segmentation_2D_prediction_only.ipynb) a user-friendly notebook for 2D segmentation written by [@pr4deepr](https://github.com/pr4deepr)
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*[](https://colab.research.google.com/github/HenriquesLab/ZeroCostDL4Mic/blob/master/Colab_notebooks/Beta%20notebooks/Cellpose_2D_ZeroCostDL4Mic.ipynb) a user-friendly [ZeroCostDL4Mic](https://github.com/HenriquesLab/ZeroCostDL4Mic) notebook that includes training cellpose models, written by [@guijacquemet](https://github.com/guijacquemet)
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*[run_cellpose3.ipynb](https://github.com/MouseLand/cellpose/blob/main/notebooks/run_cellpose3.ipynb)[](https://colab.research.google.com/github/MouseLand/cellpose/blob/main/notebooks/run_cellpose3.ipynb): run image restoration and segmentation with Cellpose3
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*[run_cyto3.ipynb](https://github.com/MouseLand/cellpose/blob/main/notebooks/run_cellpose3.ipynb)[](https://colab.research.google.com/github/MouseLand/cellpose/blob/main/notebooks/run_cyto3.ipynb): segment with the new super-generalist "cyto3" model
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*[run_cellpose_2.ipynb](https://github.com/MouseLand/cellpose/blob/main/notebooks/run_cellpose_2.ipynb)[](https://colab.research.google.com/github/MouseLand/cellpose/blob/main/notebooks/run_cellpose_2.ipynb): train your own models with Cellpose2
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*[run_cellpose_GPU.ipynb](https://github.com/MouseLand/cellpose/blob/main/notebooks/run_cellpose_GPU.ipynb)[](https://colab.research.google.com/github/MouseLand/cellpose/blob/main/notebooks/run_cellpose_GPU.ipynb): run Cellpose segmentation in 2D and 3D
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*[](https://colab.research.google.com/github/MouseLand/cellpose/blob/main/notebooks/Cellpose_cell_segmentation_2D_prediction_only.ipynb): a user-friendly notebook for 2D segmentation written by [@pr4deepr](https://github.com/pr4deepr)
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*[](https://colab.research.google.com/github/HenriquesLab/ZeroCostDL4Mic/blob/master/Colab_notebooks/Beta%20notebooks/Cellpose_2D_ZeroCostDL4Mic.ipynb): a user-friendly [ZeroCostDL4Mic](https://github.com/HenriquesLab/ZeroCostDL4Mic) notebook that includes training cellpose models, written by [@guijacquemet](https://github.com/guijacquemet)
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:triangular_flag_on_post: All models in Cellpose, except `yeast_BF_cp3`, `yeast_PhC_cp3`, and `deepbacs_cp3`, are trained on some amount of data that is **CC-BY-NC**. The Cellpose annotated dataset is also CC-BY-NC.
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@@ -180,7 +179,7 @@ python -m cellpose
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The first time cellpose runs it downloads the latest available trained model weights from the website.
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You can now **drag and drop** any images (*.tif, *.png, *.jpg, *.gif) into the GUI and run Cellpose, and/or manually segment them. When the GUI is processing, you will see the progress bar fill up and during this time you cannot click on anything in the GUI. For more information about what the GUI is doing you can look at the terminal/prompt you opened the GUI with. For example data, see [website](https://www.cellpose.org) or this [zip file](https://www.cellpose.org/static/images/demo_images.zip). For best accuracy and runtime performance, resize images so cells are less than 100 pixels across.
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You can now **drag and drop** any images (*.tif, *.png, *.jpg, *.gif) into the GUI and run Cellpose, and/or manually segment them. When the GUI is processing, you will see the progress bar fill up and during this time you cannot click on anything in the GUI. For more information about what the GUI is doing you can look at the terminal/prompt you opened the GUI with. For example data, see [website](https://www.cellpose.org) or this [zip file](https://www.cellpose.org/static/images/demo_images.zip). For best accuracy and runtime performance, resize images so cells are less than 100 pixels across. If you have 3D tiffs, open the GUI with `python -m cellpose --Zstack
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## Step-by-step demo
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### 3D segmentation
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For multi-channel, multi-Z tiff's, the expected format is Z x channels x Ly x Lx.
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For multi-channel, multi-Z tiff's, the expected format is Z x channels x Ly x Lx. Open the GUI for 3D stacks with `python -m cellpose --Zstack`.
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### Download of pretrained models
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The models will be downloaded automatically from the [website](https://www.cellpose.org) when you first run a pretrained model in cellpose. If you are having issues with the downloads, you can download them from this [google drive zip file](https://drive.google.com/file/d/1zHGFYCqRCTwTPwgEUMNZu0EhQy2zaovg/view?usp=sharing), unzip the file and put the models in your home directory under the path .cellpose/models/, e.g. on Windows this would be C:/Users/YOUR_USERNAME/.cellpose/models/ or on Linux this would be /home/YOUR_USERNAME/.cellpose/models/, so /home/YOUR_USERNAME/.cellpose/models/cyto_0 is the full path to one model for example. If you cannot access google drive, the models are also available on baidu: Link:https://pan.baidu.com/s/1CARpRGCBHIYaz7KeyoX-fg ; Fetch code:pose ; thanks to @qixinbo!
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The models will be downloaded automatically from the [website](https://www.cellpose.org) when you first run a pretrained model in cellpose. If you are having issues with the downloads, you can download them from this [google drive zip file](https://drive.google.com/file/d/1zHGFYCqRCTwTPwgEUMNZu0EhQy2zaovg/view?usp=sharing), unzip the file and put the models in your home directory under the path .cellpose/models/, e.g. on Windows this would be C:/Users/YOUR_USERNAME/.cellpose/models/ or on Linux this would be /home/YOUR_USERNAME/.cellpose/models/, so /home/YOUR_USERNAME/.cellpose/models/cyto_0 is the full path to one model for example. If you cannot access google drive, the models are also available on baidu: Link:https://pan.baidu.com/s/1CARpRGCBHIYaz7KeyoX-fg ; Fetch code:pose ; thanks to @qixinbo!
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